Free Triiodothyronine (Free T3)

Free T3 shows the active thyroid hormone available to your cells and helps clarify thyroid function.

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Test Summary

Sample Type 
Blood
Required Blood
1 mL
Analysis Type
Serum
Collection method
In person at the lab; at-home where available

Why test

Free Triiodothyronine (Free T3)

?

Clinicians order Free T3 when thyroid symptoms don’t match other tests, or to monitor therapy that includes liothyronine (T3). It helps assess suspected overactive or underactive thyroid and T4-to-T3 conversion. It’s useful when TSH or Free T4 are borderline but symptoms persist. You can test this marker with Aniva across Germany and Finland.

What is

Free Triiodothyronine (Free T3)

?

Clinicians order Free T3 when thyroid symptoms don’t match other tests, or to monitor therapy that includes liothyronine (T3). It helps assess suspected overactive or underactive thyroid and T4-to-T3 conversion. It’s useful when TSH or Free T4 are borderline but symptoms persist. You can test this marker with Aniva across Germany and Finland.

What insights will i get from

Free Triiodothyronine (Free T3)

?

High: May suggest an overactive thyroid, too much T3 medication, or thyroid inflammation. Often reviewed with TSH and Free T4 to confirm the pattern.
Low: May suggest an underactive thyroid, reduced T4-to-T3 conversion, or effects of illness or certain medicines. If results surprise you, discuss timing, supplements, and meds, then consider a repeat test with your clinician.

Sample type & collection

  • Sample: Blood
  • Fasting: No
  • Best timing: Morning preferred for consistency
  • Collection: Venipuncture
  • Typical volume: 1 mL
  • Analysis: Immunoassay (serum)
  • Prep tips: Avoid high-dose biotin for 48 hours; note thyroid meds
  • Turnaround: 1–3 business days

Free Triiodothyronine (Free T3)

is best interpreted with:

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Free Triiodothyronine (Free T3)

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Limits & interferences with

Free Triiodothyronine (Free T3)

:

Common factors that can skew results include high-dose biotin supplements, recent thyroid medication (levothyroxine or liothyronine), antithyroid drugs, amiodarone, glucocorticoids, and heparin. Acute illness, pregnancy, estrogen therapy, severe calorie restriction, and timing differences can also shift Free T3. Aim for steady routines, and tell the lab about all medicines and supplements.

Special situations: results during pregnancy, severe illness, or soon after contrast dye may need confirmation with repeat testing or alternate methods.

Questions about

Free Triiodothyronine (Free T3)

:

What does a Free T3 result mean? It shows the amount of active thyroid hormone available to your cells. It’s interpreted with TSH and Free T4 for a fuller picture.

Do I need to fast for this test? No. Fasting is not required for Free T3.

What can affect my Free T3 level? Biotin supplements, thyroid medicines, amiodarone, steroids, heparin, and acute illness can shift results. Tell your clinician about all meds and supplements.

How often should I test Free T3? It depends on your situation. Monitoring is usually tied to symptoms, treatment changes, or follow-up after dose adjustments.

How quickly will I get results? Most labs report within 1–3 business days.

What should I discuss with my clinician? Share symptoms, medication timing, supplement use (especially biotin), pregnancy plans, and related test results like TSH and Free T4.

Sources:

  • American Thyroid Association — Thyroid Function Tests. (2023). https://www.thyroid.org/thyroid-function-tests/
  • ARUP Consult — Thyroid Function Testing. (2023). https://arupconsult.com/content/thyroid-function-testing
  • MedlinePlus — T3 (Triiodothyronine) Test. (2024). https://medlineplus.gov/lab-tests/t3-triiodothyronine-test/
  • FDA — Biotin May Interfere with Lab Tests. (2019). https://www.fda.gov/medical-devices/safety-communications/update-fda-warns-biotin-may-interfere-lab-tests-fda-safety-communication
  • Mayo Clinic Laboratories — Triiodothyronine (T3), Free, Serum. (2023). https://www.mayocliniclabs.com/test-catalog/Overview/62083
Medical disclaimer: This content is for informational purposes only and is not medical advice. Always discuss results with a qualified healthcare professional.

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