Total Testosterone

Measures the overall testosterone in your blood, a key hormone for sexual health and vitality.

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Total Testosterone
 test

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Test Summary

Sample Type 
Blood
Required Blood
1 mL
Analysis Type
Serum
Collection method
In person at the lab; at-home where available

Why test

Total Testosterone

?

Clinicians order total testosterone when symptoms suggest low or high androgen activity. It helps guide evaluation of sexual health, fertility, menstrual changes, and unexplained fatigue or muscle changes. Results often inform repeat morning testing and related hormone checks for context. You can test this marker with Aniva across Germany and Finland.

What is

Total Testosterone

?

Clinicians order total testosterone when symptoms suggest low or high androgen activity. It helps guide evaluation of sexual health, fertility, menstrual changes, and unexplained fatigue or muscle changes. Results often inform repeat morning testing and related hormone checks for context. You can test this marker with Aniva across Germany and Finland.

What insights will i get from

Total Testosterone

?

High: May reflect supplementation, certain medicines, or rare endocrine conditions; in women, can relate to acne, hair growth, or irregular periods.
Low: Can occur with aging, poor sleep, obesity, chronic illness, or medicines like opioids or steroids. Recheck a morning sample and consider related hormones (free testosterone, SHBG, LH/FSH) for a fuller picture. Simple steps like better sleep, regular activity, and moderating alcohol can support healthy levels.

Sample type & collection

  • Sample: Blood
  • Fasting: No
  • Best timing: Morning (7–10 am); repeat on a different morning if needed
  • Collection: Venous blood draw
  • Typical volume: 1 mL
  • Analysis: Serum; LC-MS/MS preferred for accuracy
  • Prep tips: Test in the morning; avoid high-dose biotin 24–72 hours if advised; note hormones and medicines
  • Turnaround: 1–3 business days

Total Testosterone

is best interpreted with:

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Total Testosterone

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Limits & interferences with

Total Testosterone

:

Common factors that can skew results include time of day (morning is highest), recent meals, hard exercise, alcohol use, poor sleep, and acute illness. Medicines and supplements—testosterone, anabolic steroids, antiandrogens, glucocorticoids, opioids, and high-dose biotin—may alter or interfere with measurements. Oral estrogen therapy and pregnancy raise SHBG, which can change total levels; dehydration and lab method differences can also play a role.

Special situations (when to confirm or adjust): adolescents, shift workers, pregnancy, recent major illness, or hormone therapy—repeat a morning test and add related hormones.

Questions about

Total Testosterone

:

What does a total testosterone result mean? It shows your overall testosterone amount. Age, sex, time of day, and symptoms help interpret it. Abnormal results are often confirmed with a repeat morning test.

Do I need to fast for this test? Fasting is usually not required. A morning sample is recommended; follow your clinician’s advice if they prefer fasting for consistency.

When should I test and how often? Test in the morning, ideally 7–10 am. If levels are borderline or unexpected, repeat on a different morning; future testing depends on symptoms or treatment plans.

What can affect my results? Hormone therapy, anabolic steroids, opioids, glucocorticoids, oral estrogen, high-dose biotin, illness, poor sleep, and hard exercise can shift levels. Share all medicines and supplements with your clinician.

How long do results take? Most labs report within 1–3 business days, depending on method and location.

What should I discuss with my clinician? Your symptoms, timing of the sample, medications, supplements, and fertility goals. Ask whether to check free testosterone, SHBG, LH/FSH, or other related tests.

Sources:

  • Endocrine Society — Testosterone Therapy in Men with Hypogonadism. (2018). https://www.endocrine.org/clinical-practice-guidelines/testosterone-therapy-in-men-with-hypogonadism
  • American Urological Association — Testosterone Deficiency Guideline. (2018). https://www.auanet.org/guidelines-and-quality/guidelines/testosterone-deficiency-guideline
  • MedlinePlus — Testosterone Test. (2023). https://medlineplus.gov/lab-tests/testosterone-test/
  • ARUP Consult — Male Hypogonadism. (2024). https://arupconsult.com/content/male-hypogonadism
  • FDA — Biotin may interfere with lab tests. (2019). https://www.fda.gov/medical-devices/safety-communications/fda-safety-communication-biotin-may-interfere-lab-tests-fda-safety-communication
Medical disclaimer: This content is for informational purposes only and is not medical advice. Always discuss results with a qualified healthcare professional.

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